Let me start with introducing myself. My name is Dries Verhelst and I’m currently studying biochemistry at GroupT Leuven. One of the activities I have to do to complete my master this year is take an internship and write a thesis about it.
For my thesis I’ve been giving the chance to do my internship at the Molecular Imaging and Photonics faculty of the Catholic University of Leuven (KULeuven). This group of scientists specialize themselves in expressing fluorescent proteins (fluorophores) and use these molecules for ‘superresolution fluorescence imaging’ . This technique(s) overcomes the diffraction resolution barrier (caused by light) used in classical microscopes to reveal more details. If you want more information about these techniques you can allways ask me or pm me about it.
The actual subject of my thesis is to create/find a “positive switching” mutant of a reversible switchable fluorescent protein called rsGreen (based on EGFP) and characterize this protein for further use. If you want more details or information regarding this subject, again, don’t hesitate to PM me or ask questions. I will be happy to answer them.